Fig. 6.

Mutational mapping. The SpoIIE chains are shown in ribbon format with cyan and light-blue coloring indicating the different chains of the domain-swapped dimer. The dark-blue color represents the flap region. In cylinder representation with atoms colored according to type are side chains of residues identified in genetic studies of SpoIIE function. Side chains with green carbon atoms indicate residues whose mutation suppresses the effect of spoIIE48 or the Gln483-to-Ala mutation. The coral-colored Leu646 and Gly609 (shown as a C^α sphere) are the sites of mutation in spoIIE64 and spoIIE71, respectively.