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Young (0 day) sex-separated pupae were injected with dsRNA targeting all isoforms of TcdsxC (f1+f2+f3), Tcdsxf1+ Tcdsxf2 (f1+f2), Tcdsxf2 alone or malE. Ovaries or testis were dissected on 5th day PAE, stained with acridine orange and photographed at 4X (ovary) or 10X magnification (testis) using fluorescence microscope.
