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. Author manuscript; available in PMC: 2013 Mar 20.
Published in final edited form as: Neuron. 2012 Sep 20;75(6):1067–1080. doi: 10.1016/j.neuron.2012.07.009

Fig. 6. nElavl-dependent regulation of the brain enzyme glutaminase.

Fig. 6

(A) The two mRNA isoforms of the glutaminase gene (Gls) and nElavl binding sites are shown. Individual colors depict different experiments. Alternative use of a 3’ splice site generates two Gls isoforms with different 3’ terminal coding sequences and 3’ UTRs. Gls-s and Gls-l refer to short and long isoforms, respectively. (B) RT-PCR amplification of the two Gls isoforms in WT and Elavl3-/-;Elavl4-/- cortex of age P0 mice. (C) Western blot analysis of the two Gls isoforms in littermate WT and Elavl3-/-;Elavl4-/- cortex of age P0 mice. Each lane represents an independent mouse. (D) Q-PCR quantification of the abundance of two Gls mRNA isoforms in littermate WT and Elavl3-/-;Elavl4-/- cortex of age P0 mice. (E) Quantification of data shown in panel (C). (F) Quantification of total glutamate levels in cortex of 3 WT and 3 Elavl3-/-;Elavl4-/- littermate age P0 mice are presented. Glutamate levels in WT samples are normalized to 100% in the Y-axis. * denotes p<0.01 (t-test). Error bars denote standard deviation. See also Fig.S3-4 and Table S9.