Table II.
Effect of MeSA on SA concentration and TMV-lesion size in wild-type and NahG tobacco plants
Plant | SA
|
Lesion Diameter | |
---|---|---|---|
d 0 | d 6 | ||
ng g−1 fresh wt | mm | ||
Wild type | 34 ± 1 | 6546 ± 683 | 0.852 ± 0.04 |
NahG | 45 ± 4 | 84 ± 17 | 1.785 ± 0.08 |
Tobacco plants were enclosed in gas-tight glass chambers to which 250 μg L−1 MeSA was supplied daily. After 6 d of incubation the plants were sampled and analyzed for SA content. Each value is the mean ± se of three replicates. Plants were then inoculated with TMV, and the diameter of the TMV-induced lesions was measured 72 h postinoculation. Data represent the mean diameter ± se of at least 30 lesions per plant. The experiment was repeated with similar results. The diameters of TMV lesions in untreated wild-type and NahG plants were 1.328 ± 0.09 and 1.800 ± 0.11 mm, respectively.