Table 1.

Physical properties and behavior in biological systems of multifunctional reagent modules (5a, 5b), and unPEGylated (6a, 6b), and PEGylated Probes (7a, 7b). Bold = 5 kDa PEG.

	F3: 5 kDa PEG	Target Pept.	MW (Da)	MS Obs. (Da)	Quant. Yield, Fig. 2b	Fluor. Life-time (ns) Fig. 2c	Volume By FPLC (kDa) Fig. 2e	Cell Fluor. (a.u.) Fig. 3b	IM Inject. Fig. 3c, 3d
5a	No	None	1667.0	1666.2	0.061±0.006	0.31±0.04	0.4	79.7±4.3
5b	Yes	None	6805.1	6850 (peak)	0.17±0.008	0.66±0.02	20	3.1±0.2
6a	No	RGD	2848.3	2848.3	0.082±0.004	0.52±0.05	0.4	26.7±0.6
6b	No	RAD	2862.4	2862.2	0.081±0.004	0.50±0.02	0.4	20.4±0.4	Retained
7a	Yes	RGD	7987.4	7980 (peak)	0.20±0.01	0.81±0.02	25	9.7±0.3
7b	Yes	RAD	8001.4	8000 (peak)	0.20±0.01	0.80±0.03	25	3.2±0.2	Elim.
CyAL5.5	No	None	800.01	799.31	0.08±0.004	0.40±0.03	N.O.	N.O