Table 2.
Effect on bacterial growth of isolates from BBD and SML to purified microcystin (MC-LR) at 1, 100, and 500 µg l−1. Growth was compared to that of controls (no microcystin) for each isolate for each experiment (N = 3 experiments, each with triplicate incubations).
| Bacterial isolate Source Strain designation |
Identification (closest GenBank sequence, % similarity) |
Response/[MC]a | P value b |
|---|---|---|---|
| BBD | |||
| BBD-216-1a | Vibrio harveyi (AY750576, 99%) | - | |
| BBD-216-4a | Methylarcula sp. (AJ534208, 96%) | S/100 µg l−1 | p < 0.02 |
| BBD-216-3d | Bacillus megaterium (AJ717381, 99%) | - | |
| BBD-216-4f | Marinobacter sp. (AY196982, 99%) | - | |
| BBD-216-4g | M. aquaeolei (AJ000726, 99%) | - | |
| BBD-216-4i | Idiomarina sp. (AB167036, 98%) | S/ 1 µg l−1 | p < 0.01 |
| BBD-217-1c | Photobacterium eurosenbergii (AJ842346, 99%) | S/500 µg l−1 | p < 0.05 |
| BBD-217-1a | Bacillus cereus (AY305275, 99%) | - | |
| BBD-217-2d | Vibrio harveyi (AY750576, 99%) | I/100 µg l−1 | p < 0.05 |
| BBD-217-2g | Alteromonas sp. (AY626838, 99%) | - | |
| SML | |||
| H-1-2 | not identified | I/ 1 µg l−1 | p < 0.0005 |
| H-1-3 | “ | I/500 µg l−1 | p < 0.05 |
| H-1-5 | “ | - | |
| H-1-7 | “ | I/ 1 µg l−1 | p = 0.005 |
| H-1-8a | “ | - | |
| H-1-8b | “ | I/ 1 µg l−1 | p = 0.001 |
| S/500 µg l−1 | p < 0.005 | ||
| H-1-9 | “ | - | |
| H-1-10 | “ | - | |
| H-1-11 | “ | - | |
| H-1-12 | “ | - | |
| H-1-13 | “ | - | |
| H-1-16 | “ | I/ 1 µg l−1 | p < 0.005 |
| S/100 µg l−1 | p = 0.0002 | ||
| Known coral pathogens | |||
| Aurantimonas coralicida | na | - | |
| Serratia marcescens | na | S/500 µg l−1 | p < 0.05 |
| Vibrio shiloi | na | S/ 1 µg l−1 | p < 0.01 |
a
Response designated as S (stimulation), I (inhibition) - (no effect)
b
two sample t-Test, assuming equal variances
na = not applicable