Table 1.
Gene | Primer sequences (sense/antisense) | Efficiency | Annealing temperature (°C) | Product length (bp) |
---|---|---|---|---|
GAPDH |
5′;-TGGTATCGTGGAAGGACTCA-3′; |
1.93 |
67 |
132 |
5′;-CCAGTAGAGGCAGGGATGAT-3′; | ||||
DEFA1/3 |
5′;-ATGAGGACCCTCGCCATCCTTGCT-3′; |
2.17 |
69 |
285 |
5′;-TCAGCAGCAGAATGCCCAGCGTCTTCCC-3′; | ||||
DEFA4 |
5′;-GTCTGCTCTTGCAGATTAGTATTCTG-3′; |
1.98 |
69 |
105 |
5′;-TTAATCGACACGCGTGCAGCAGTAT-3′; |
PCR efficiencies for every set of primers were determined with dilution series of primer specific cloned PCR-products at the corresponding annealing temperature. Efficiency stands for the performance to amplify a cDNA template. Efficiency values of 2.0 means an amplification of 100%.