Table 1.

Primer sequences with corresponding annealing temperatures, efficiencies, and product length in basepairs (bp) used for realtime PCR

Gene	Primer sequences (sense/antisense)	Efficiency	Annealing temperature (°C)	Product length (bp)
GAPDH	5′;-TGGTATCGTGGAAGGACTCA-3′;	1.93	67	132
	5′;-CCAGTAGAGGCAGGGATGAT-3′;
DEFA1/3	5′;-ATGAGGACCCTCGCCATCCTTGCT-3′;	2.17	69	285
	5′;-TCAGCAGCAGAATGCCCAGCGTCTTCCC-3′;
DEFA4	5′;-GTCTGCTCTTGCAGATTAGTATTCTG-3′;	1.98	69	105
	5′;-TTAATCGACACGCGTGCAGCAGTAT-3′;

PCR efficiencies for every set of primers were determined with dilution series of primer specific cloned PCR-products at the corresponding annealing temperature. Efficiency stands for the performance to amplify a cDNA template. Efficiency values of 2.0 means an amplification of 100%.