Fig. 1.

Image of a peptide microarray. Small section from a peptide array used for identification of different peptide epitopes, including the ones in Fig. 2A. The peptides were synthesized in quadratic fields defined by 2 × 2 mirrors (each mirror measuring 10 μm × 10 μm). One-mirror-wide empty regions separated the peptide fields. The fields were visualized via incubation with relevant rabbit antibodies followed by Alexa488-conjugated goat anti-rabbit IgG. The section shown corresponds to ca. 0.15% of the area of the entire array. Note that peptide synthesis at single mirror resolution can be observed.