Fig. 3.

CR results in broad and substantial reductions in hepatic protein synthesis and breakdown rates and prolongation of protein half-lives. A, Direct comparison of half-lives for all observed proteins shows that CR (black) prolonged the half-lives relative to AL (open) for 85% of proteins. B, Absolute synthesis rates for each protein were calculated from the replacement rate and the SILAM concentration measurements. Differences between CR and AL mice were enhanced on this absolute scale, with CR mice producing substantially less of almost all proteins.