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. 2012 Sep 6;11(12):1690–1708. doi: 10.1074/mcp.M112.019778

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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Fig. 1. — A, Immunofluorescence analysis of cytoskeleton changes in Swiss 3T3 fibroblasts induced by long-term stimulation with PDGF, IGF-1, EGF and their combinations. Focal visualized by fluorescence staining with anti-vinculin antibody and rhodamine-phalloidin staining, respectively. Dendritic protrusions are indicated by arrows (middle column). Note the dominance of PDGF in costimulation experiments, resulting in development of an elongated cell shape with retractile dendritic protrusions and disruption of stress fibers (SF) and focal adhesion (FA) complexes. B, Immunofluorescence analysis of temporal changes in the actin cytoskeleton during long-term GF-treatment. Actin filaments were stained with rhodamine phalloidin. In the PDGF-treated cells, SF and FA had disappeared by 6 h, dendritic protrusions began to appear after 12 h and the development of an elongated, motile cytoskeleton was fully accomplished by 18 h treatment. C, Changes in focal adhesion density and cell surface area in response to growth factor stimulation.