Fig. 5.

A, ³⁵S-autoradiograms demonstrating kinase inhibitor-induced modulation in PDGF-regulated protein synthesis at the 18 h time point. Newly synthesized EF2 isoforms are boxed by white rectangles. The abundance of newly synthesized in general were less affected by inhibition of MEK or TOR than by inhibition of the PI3K/Akt pathway in cells exposed to long-term PDGF treatment. Newly synthesized eEF2 isoforms are indicated by white boxes. B, Hierarchical clustering analysis of protein synthesis in cells treated with EGF or PDGF for 6, 12, and 18 h, or cells treated with PDGF in the presence of LY290042 for 18 h. The resulting 2DE images were clustered according to similarities between all radiolabeled protein spots. C, Clustering analysis demonstrating that cells treated with PDGF for 18 h in the presence of the MEK inhibitor PD98059 clustered in proximity to images from cells exposed to two growth factors.