Fig. 3.

Reduction of the abundance of LAT2 by alkylphospholipids. A, Western blot validation (total cell extract) of the effect of ODPC on LAT2 in NB4 and U937 cells. K562 does not express LAT2. B, Western blots (total cell extract) show the reduction of LAT2 after treatment with perifosine (10 and 25 μm) in NB4 and U937 cells. C, D, Western blotting of LAT2 in cell fractions of control and ODPC-treated NB4 cells (25 μm for 3 h). The successive detergent fractionation method was used in C, and the sucrose density gradient centrifugation method was used in D. DSM, detergent soluble membranes; DRM, detergent resistant membranes.