Fig. 4.

Chemically induced perturbations of lipid raft structure mimic or potentiate the effect of ODPC on LAT2. Inhibition of protein palmitoylation by 100 μm 2-bromopalmitic acid (2-BrPA) has an effect on LAT2 abundance (A) and apoptosis induction (B) that is very similar to that of 25 μm ODPC treatment. NB4 cells were exposed to 2-BrPA. LAT2 abundance was evaluated via Western blot, and apoptosis induction via annexin-V and propidium iodide staining by flow cytometry at the indicated times. C, cholesterol depletion by methyl-β-cyclodextrin (MCD) increases the effect of ODPC on LAT2 abundance; however, MCD has a very limited effect when used alone. NB4 cells were exposed to MCD (2.5 mg/ml) in serum-free medium for 30 min, and subsequently MCD was washed out and complete medium was restored. LAT2 abundance was evaluated via Western blot at the indicated times.