Table II. Proteolytic peptides of ^Cab−SG−MIH identified by bottom-up de novo sequencing.

Obsd. m/z	Charge state	Calcd. MW	Sequencea	Proteolytic peptide	Mascot ion scoreb	Protease
828.49	2	1654.82	RVINDDCPNLIGNR	MIH [1–14]	65	Trypsin
750.40	2	1498.71	VINDDCPNLIGNR	MIH [2–14]	70	Trypsin
1010.11	2	2017.99	VINDDCPNLIGNRDLYK	MIH [2–18]	76	Trypsin
928.50	2	1854.84	KVEWICEDCSNIFR	MIH [19–32]	90	Trypsin
864.48	2	1726.74	VEWICEDCSNIFR	MIH [20–32]	53	Trypsin
512.30	2	1022.46	NTGMATLCR	MIH [33–41]	50	Trypsin
1200.16	2	2398.05	KNCFFNEDFLWCVYATER	MIH [42–59]	79	Trypsin
1136.11	2	2269.96	NCFFNEDFLWCVYATER	MIH [43–59]	95	Trypsin
590.96	2	1769.85	RVINDDCPNLIGNRD	MIH [1–15]	49	Glu-C

^a Fixed carbamidomethyl is on cysteine.

^b Matching to homologous sequence.