Table 6.
Serum Cytokine Profile 1 Day post Oropharyngeal Aspiration of 100 μg MWCNTs (pg/ml)
Cytokine/Treatment | SS | C-grade | COOH | N-doped |
---|---|---|---|---|
Eotaxin |
306.4 ± 75.0 |
276.9 ± 42.3 |
903.8 ± 278.6* |
302.6 ± 22 |
IL-5 |
16.3 ± 1.1 |
30.3 ± 10.0 |
11.6 ± 3.0 |
51.2 ± 28.3 |
IL-6 |
9.2 ± 1.3 |
16.1 ± 7.8 |
137.8 ± 66.0 |
42.4 ± 26.4 |
IL-10 |
8.5 ± 1.9 |
8.6 ± 3.4 |
24.6 ± 15.4 |
125.8 ± 118.1 |
IL-12 (p40) |
17.5 ± 3.6 |
20.3 ± 5.3 |
16.3 ± 2.2 |
12.6 ± 2.2 |
IL-12 (p70) |
35 ± 5.9 |
55.3 ± 34.9 |
22.9 ± 3.1 |
516.7 ± 464.5 |
IL-13 |
446.3 ± 41.6 |
326.9 ± 38.2 |
365.3 ± 45.6 |
848.3 ± 490.0 |
KC |
173.2 ± 32.6 |
97.1 ± 7.1 |
645.1 ± 441.3 |
183.5 ± 53.1 |
MCP-1 |
39.6 ± 12.3 |
36.6 ± 8.6 |
46.6 ± 6.0 |
136.1 ± 104.4 |
MIP1α |
38.2 ± 9.3 |
71.1 ± 4.5 |
42.0 ± 4.4 |
128.1 ± 86.9 |
MIP-1β |
129.0 ± 11.4 |
70.2 ± 22.3 |
82.8 ± 29.6 |
136.2 ± 48.8 |
MIP-2 |
155.4 ± 27.7 |
86.4 ± 43.8 |
209.1 ± 38.9 |
102.4 ± 26.4 |
RANTES |
30.7 ± 5.8 |
36.1 ± 6.4 |
57.5 ± 12.0 |
65.9 ± 38.5 |
†IL-1β |
33.25 ± 3.83 |
222.86 ±114.71 |
127.66 ± 45.32 |
47.25 ± 7.36 |
†TNF-α | N.D. | N.D. | N.D. | N.D. |
Serum cytokine profile by Lincoplex Cytokine/Chemokine 20 plex kit 1 day post oropharyngeal aspiration of three forms of MWCNTs at 100 μg and vehicle control SS (10% surfactant saline). Data reported as mean ± SEM for n = 4. * indicates significance from SS, P ≤ 0.05.
† IL-1β and TNF-α profile 1 day post oropharyngeal aspiration of three forms of MWCNTs at 100 μg and vehicle control SS (10% surfactant saline) by ELISA. Data reported as mean ± SEM for n = 6. N.D. not detected, P ≤ 0.05 by one way- ANOVA.