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Effect of proteasome inhibitor on UT-A1 endocytosis. UT-A1-MDCK cells were pretreated with 10 μM MG132 or 10 μM lactacystin (Lact) for 1 h and then treated with or without 10 μM FSK for 3 h. UT-A1 internalization was examined by biotinylation and MesNa treatment was as above. The bands were quantified (n = 3). The internalized UT-A1 was normalized to the UT-A1 from total lysates. The relative density of the control (without any treatment) was set as 1. *P < 0.05. **P < 0.01.
