Fig. 4.

Pharmacological PDE blockade with 10 μM IBMX reduced the intracellular Ca²⁺ levels in freshly isolated human UBSM cells. A: original recording illustrating that PDE blockade decreased the cellular Ca²⁺ levels in a single UBSM cell shown as a ratio of fura-2 fluorescence emission intensities at 510 nm with excitation at 340 and 380 nm. B: summary data indicating that PDE blockade significantly decreased the intracellular Ca²⁺ levels (n = 22, N = 4; *P < 0.05).