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. 2012 Dec 10;199(6):931–949. doi: 10.1083/jcb.201205115

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© 2012 Ricke et al.

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Figure 2. — Bub1 kinase activity is required for chromosome alignment and maintenance of chromosomal stability. (A) Karyotype analysis of passage 5 MEFs. Statistics indicate comparison with wild type. (B) Representative images for chromosome segregation defects by live-cell imaging. White arrowheads shows misaligned chromosomes. Bar, 10 µm. (C) Live-cell imaging of chromosome segregation defects in primary MEFs. Statistics indicate comparison with wild type. (D) Analysis of mitotic duration in Bub1^+/+ and Bub1^KD/KD MEFs after colcemid addition (n = 3 or more lines per genotype). Error bars indicate SEM. The time at which 50% of the cells exited mitosis was compared. Statistics indicate comparison with wild type. (E) Same as D but with 0.5 µM taxol. chr., chromosome. *, P < 0.5; **, P < 0.1; ***, P < 0.001.