Figure 4.

GO categorization of the 6-shogaol-regulated proteins and the validation of differentially expressed proteins. Based on the PANTHER classification and gene ontology (GO) categorization, molecular function (a) and biological process (b) of the corresponding identified proteins were classified. (c) The differentially expressed proteins Annexin A1, cofilin, and calreticulin were validated by Western blot. Control and 6-shogaol marked above the panel represent the control cells and cells treated with 15 μM of 6-shogaol for 24 h. Tubulin was used to normalize protein loading. Each blot is the representative result of three independent experiments.