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. Author manuscript; available in PMC: 2013 May 11.
Published in final edited form as: Mol Cell. 2012 Mar 29;46(3):351–361. doi: 10.1016/j.molcel.2012.02.018

Figure 2. Skp2 Interacts with ATM and MRN Complex, and Its E3 Ligase Activity Is Required for the Recruitment of ATM to DNA Damage Foci.

Figure 2

(A) 293 cells with GFP or Skp2 knockdown were treated with or without IR (10 Gy) and harvested for coimmmunoprecipitation assay 5 min after IR treatment.

(B) Skp2–/– MEFs restored with mock, Skp2, and Skp2-NES were treated with IR (3 Gy) and harvested for IF analysis 5 min after IR treatment.

(C) Skp2–/– MEFs restored with mock, Skp2, and Skp2-NES were treated with IR (3 Gy) and harvested for western blot analysis 5 min after IR treatment.