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. Author manuscript; available in PMC: 2013 May 11.
Published in final edited form as: Mol Cell. 2012 Mar 29;46(3):351–361. doi: 10.1016/j.molcel.2012.02.018

Figure 3. Skp2 Is Required for the Interaction of NBS1 with ATM upon DNA Damage.

Figure 3

(A) 293 cells with GFP or Skp2 knockdown were treated with IR (10 Gy) and harvested for coimmunoprecipitation assay at various time points, followed by western blot analysis.

(B) 293 cells with GFP or Skp2 knockdown were treated with or without IR (10 Gy) and harvested for coimmmunoprecipitation assay at various time points.

(C) 293T cells were transfected with Xp-Skp2 and various Flag-NBS1 truncation mutants as indicated, and cells were harvested for coimmmunoprecipitation assay, followed by western blot analysis.