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. Author manuscript; available in PMC: 2012 Dec 10.
Published in final edited form as: Biochem Biophys Res Commun. 2011 Jan 15;405(3):445–449. doi: 10.1016/j.bbrc.2011.01.050

Figure 3.

Figure 3

Whole cell radioligand binding analysis with 2 nM [3H]SCH-23390 on HA- wild-type D1 receptor or HA-D1-C347A/C351A receptor-expressing cells exposed to 10 μM SKF 81297. D1-C347A/C351A receptor expressing cells were pre-treated with either vehicle (control) or 0.45 M sucrose, 0.25 mg/ml concanavalin A, 2% methyl-β-cyclodextrin or 30 μM H89 and wild-type D1 receptor expressing cells were pre-treated with either vehicle (control) or 2% methyl-β-cyclodextrin for 30 min prior to agonist stimulation with SKF 81297 (10 μM) for an additional 30 min. The results are expressed as the mean % internalization ± S.E.M. of 3 independent experiments. Significance at p<0.05, p<0.01, and p<0.001 compared to control values is denoted by *, **, and ***, respectively.