Table 1.

Relative fluorescence of the different immunostimulating complex (ISCOM) preparations

Sample	Relative fluorescence (mean ± SD)
ISCOM-phy	0.034 ± 0.01
ISCOM-phy7-A	0.058 ± 0.02
ISCOM-phy4-A	0.05 ± 0.01
ISCOM-phy7-A-IgGsp	16.477 ± 0.45***
ISCOM-phy4-A-IgGsp	14.03 ± 0.12***
ISCOM-9-phy-Aa	0.02 ± 0.01
ISCOM-9-phy-A-IgGspa	19.501 ± 0.31***
ISCOM-9-phy-Ab	0.03 ± 0.01
ISCOM-9-phy-A-IgGspb	12.09 ± 0.12***
ISCOM-phy7-A-IgG non-sp	0.5 ± 0.16
ISCOM-phy4-A-IgG non-sp	0.03 ± 0.01
ISCOM-9-phy-A-IgG non-spa	0.058 ± 0.02

Note:

^***P < 0.001 by the Tukey–Kramer multiple comparisons test, between the samples without specific G immunoglobulin (IgG).

Abbreviations: IgG, immunoglobulin; ISCOM-phy, ISCOM labelled with phycocyanin; ISCOM-phy 7-A, protein A bound to ISCOM-phy with compound 7; ISCOM-phy 4-A, protein A bound to ISCOM-phy with compound 4; ISCOM-phy 7-A-IgGsp, ISCOM-phy, compound 7 with protein A and Trypanosoma cruzi-specific mouse IgG; ISCOM-phy 4-A-IgGsp, ISCOM-phy, compound 4 with protein A and T. cruzi-specific mouse IgG; ISCOM-9-phy-Aa, ISCOM-phy prepared with modified cholesterol (compound 9 at a ratio of 1:1) and protein A; ISCOM-9-phy-A-IgGspa, ISCOM-phy prepared with modified cholesterol (compound 9 at a ratio of 1:1) and protein A and T. cruzi-specific mouse IgG; ISCOM-9-phy-Ab, ISCOM-phy prepared with modified cholesterol (compound 9 at a ratio of 2:3) with protein A; ISCOM-9-phy-A-IgGspb, ISCOM-phy prepared with modified cholesterol (compound 9 at a ratio of 2:3) with protein A and T. cruzi-specific mouse IgG; ISCOM-phy 7-A-IgG non-sp ISCOM-phy prepared with compound 7, protein A and nonspecific human IgG; ISCOM-phy 4-A-IgG non-sp, ISCOM-phy prepared with compound 4, protein A and nonspecific human IgG; ISCOM-9-phy-A-IgG non-sp, ISCOM-phy prepared with modified cholesterol (compound 9 at a ratio of 2:3), protein A and nonspecific human IgG.