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. Author manuscript; available in PMC: 2012 Dec 10.
Published in final edited form as: Methods Enzymol. 2009;457:63–80. doi: 10.1016/S0076-6879(09)05004-6

Figure 2.

Figure 2

32P Dose Response in Heart Mitochondria. Porcine heart mitochondria were incubated in standard conditions for 20 min where A) 1μCi B) 25μCi, C) 50μCi and D) 250μCi of 32P was added. Proteins were separated in the horizontal direction by isoelectric focusing point (pI), from pH ~4 to 10, and vertically by molecular weight, from ~150 to 10 kDa. The relative amplitude for each image was arbitrarily set.