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. Author manuscript; available in PMC: 2012 Dec 10.
Published in final edited form as: Methods Enzymol. 2009;457:63–80. doi: 10.1016/S0076-6879(09)05004-6

Figure 3.

Figure 3

32P labeling assignments in isolated heart mitochondria. Mitochondria were incubated for 20 minutes with 250 uCi of 32P. Chromatography is the same at in Figure 2. Protein assignments: 1 ATP synthase, mitochondrial F1 complex, β subunit. 2) 60 kDa heat shock protein, mitochondrial precursor. 3) NADH dehydrogenase (ubiquinone) Fe-S protein 1 (75kDa). 4) 70kDa heat shock protein, mitochondrial precursor. 5) Pyruvate dehydrogenase complex, E2 subunit. 6) ATP synthase d-chain, mitochondrial precursor. 7) Pyruvate dehydrogenase complex, E1 subunit. 8) Elongation factor Tu, mitochondrial precursor. 9) Aconitase hydratase, mitochondrial precursor. 10) Isocitrate dehydrogenase (NAD) subunit alpha, mitochondrial precursor 11) Succinyl-CoA ligase (GDPforming) alpha-chain, mitochondrial precursor. 12) Mn superoxide dismutase. 13) Creatine kinase, sarcomeric mitochondrial precursor. 14) Cytochrome c oxidase polypeptide Va, mitochondrial precursor. 15) Voltage-dependent anion channel 1. 16) ATP synthase, mitochondrial F1 complex, α subunit.