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The Effects of Dichloroacetate (DCA) and Pyruvate on 32P Labeled Heart Mitochondria. Control: Heart mitochondria incubated in standard conditions for 20 min with 32P. DCA/Pyruvate: Heart mitochondria treated with 0.1 mM DCA and 5 mM pyruvate for 1 h on ice then 32P was added during the standard 20 min incubation at 37°C. Box indicates region of the phosphorylated pyruvate dehydrogenase E1 protein.
