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Isolation and identification of CI subunits by MS. A, Representative Coomassie stained gel of CI subunits isolated from saline (Ctl) and KA-treated rats 48 h, 1 week, and 6 week post-KA injection; n = 4. Numbers 1–14 indicate band removal for PMF protein identification with MALDI TOF MS. B, Fifty percent of CI subunits were identified including the 75 kDa subunit (75) corresponding to Band 1. Additional CI subunits identified by molecular weight correspond to Bands 2–14.
