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Rapid quench experiments using CaaD and αY60W-CaaD fit to the burst equation. A) A comparison of the wild-type CaaD (⋄) and the αY60W-CaaD (▲) showing the burst of bromide followed by steady state turnover. The concentration of enzyme was 400 μM. B) Rapid quench experiment for three concentrations of αY60W CaaD: 250 μM (□), 400 μM (△), and 600 μM (○).
