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. Author manuscript; available in PMC: 2013 Nov 20.
Published in final edited form as: Biochemistry. 2012 Nov 8;51(46):9420–9435. doi: 10.1021/bi3010686

Figure 7. Global Data Fitting.

Figure 7

The αY60W-CaaD kinetic data with 9 and bromide (A–I) globally fit by simulation (solid lines) to the mechanism shown in Scheme 3 and (G) αY60W-CaaD kinetic data with 4 fit by simulation (solid lines) to give constraints on rates defined in Scheme 3 and Table 4.24 (A–E) Stopped-flow fluorescence traces (0.1–300 s) for 9 (0–10000 μM) and αY60W-CaaD. Concentrations of 9 are: 0, 50, 100, 150, 250, 550, 1000, 2000, 3000, 4000, 5000, 6000, 8000, 10000 μM. Colors corresponding to the concentrations of 9 are maintained for graphs A–E. (F) Rapid-quench of αY60W-CaaD (200, 400, and 600 μM) with 9. (G) Stopped-flow fluorescence traces for 4 (100, 200, 300, 400, 500, 750, 1500, and 2000 μM) and αY60W-CaaD. (H) Full time progress curves following 300 μM of 9 at 224 nm with increasing concentrations of bromide (0, 5000, 10000, 20000, 30000, and 50000 μM). (I) Full time progress curves following the decrease of 9 (18, 42, 63, 83, and 125 μM) at 224 nm.