Skip to main content
. Author manuscript; available in PMC: 2012 Dec 10.
Published in final edited form as: Nat Commun. 2012 Jan 10;3:614. doi: 10.1038/ncomms1629

Figure 5.

Figure 5

Figure 5

in vivo activity and determination of stoichiometry for LKγ-PNA:DNA complexes. a. Displacement of 125I-Echistatin from integrin αVβ3 on C32 cells by c(RGDfK) and DNA:PNA-D5. Kd c(RGDfK) = 6.3 × 10−8 M; Kd DNA:PNA-D5= 1.6 × 1−10 M. Error bars represent two s.d. (n=3). b. The effect of DNA:PNA-D5 on metastatic potential of B16F10 cells based on the tumor development in C57BL/6NCrmice. All mice were injected with 5×105 B16F10 cells. Error bars represent one s.d. (n=8 mice). c. Lungs of sacrificed mice after fixation with tumor lesions indicated by dark spots. The mice treated with DNA:PNA-D5 had visibly fewer tumor colonies present after 14 days compared to the control or c(RGDfK) alone. Each row corresponds to three mice out of a group of eight. d. Absorbance c(s) distributions obtained from sedimentation velocity data collected at 50 krpm and 20.0 °C for DNA:PNA-B5 at loading concentrations of 0.35 (blue), 0.78 (red) and 1.47 (green) A260. The complex was prepared using a slight excess of PNA seen at ~1.0 S.