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Accumulation of three distinct ACC synthase mRNAs in the orchid gynoecium after pollination. A, Accumulation of Ds-ACS1 and Phal-ACS2 mRNAs in the stigma. B, Accumulation of Phal-ACS3 mRNA in the ovary. Each lane contains 30 μg of total RNA. An actin cDNA probe was used as a control for equal loading of RNA. Numbers at right indicate the approximate sizes of the detected mRNAs. HAP, Hours after pollination.
