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. Author manuscript; available in PMC: 2012 Dec 10.
Published in final edited form as: J Hum Genet. 2006 Apr 1;51(5):397–406. doi: 10.1007/s10038-006-0374-9

Fig. 1.

Fig. 1

A candidate automation process. A robot, such as a CyBio CyBi-Disk, is used to aliquot primers, DNA, and master mixes to the wells of a 384-well plate, which is then placed in a thermal cycler for amplification of the genetic information. The eGene HDA-GT12 is used to detect deleted genetic regions and to measure the DNA concentration of amplicons to be sequenced. The robot then normalizes the amount of DNA in each well by adding water to some and then prepares cycle sequencing reactions. Sequencing chains are then produced in the thermal cycler, purified on the robot, and sequenced on the ABI 3,730 sequencer