A. Whole genome tiling array analysis of LSD2 binding across human chromosome 3. Vertical bars mark positions of LSD2 target sites (P<0.0001). ChIP-chip signals (MAT score) shown in log 2 scale.
B. ChIP-chip signals of LSD2 binding across CCNL1 and DNAJB11. Arrow denotes promoter orientation.
C. Distribution of LSD2 binding sites.
D. Confirmation of the preferential LSD2 binding to gene bodies. Cross-linked ChIP using anti-Flag antibody was performed from 3×Flag-LSD2 expressing HeLa (purple bars, 3×Fg-LSD2) and empty vector transduced cells (open bars, mock). Fold of enrichment relative to mock was shown. PCR primers specific to coding region or the promoter (-pro, boxed) of each gene were denoted in X-axis. Error bars, s.e.m. of triplicates from representative experiment. LSD2 did not shown significant binding to the promoter (GAPDH-pro) or an intragenic region (GAPDH-intra) of GAPDH.