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. Author manuscript; available in PMC: 2012 Dec 10.
Published in final edited form as: Mol Cell. 2010 Jul 30;39(2):222–233. doi: 10.1016/j.molcel.2010.07.008

Figure 2. CHIP-chip analysis indicates enrichment of LSD2 binding sites within the coding regions but not at promoters of genes.

Figure 2

A. Whole genome tiling array analysis of LSD2 binding across human chromosome 3. Vertical bars mark positions of LSD2 target sites (P<0.0001). ChIP-chip signals (MAT score) shown in log 2 scale.

B. ChIP-chip signals of LSD2 binding across CCNL1 and DNAJB11. Arrow denotes promoter orientation.

C. Distribution of LSD2 binding sites.

D. Confirmation of the preferential LSD2 binding to gene bodies. Cross-linked ChIP using anti-Flag antibody was performed from 3×Flag-LSD2 expressing HeLa (purple bars, 3×Fg-LSD2) and empty vector transduced cells (open bars, mock). Fold of enrichment relative to mock was shown. PCR primers specific to coding region or the promoter (-pro, boxed) of each gene were denoted in X-axis. Error bars, s.e.m. of triplicates from representative experiment. LSD2 did not shown significant binding to the promoter (GAPDH-pro) or an intragenic region (GAPDH-intra) of GAPDH.