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. Author manuscript; available in PMC: 2012 Dec 10.
Published in final edited form as: J Microsc. 2010 Jun 21;241(2):153–161. doi: 10.1111/j.1365-2818.2010.03411.x

Figure2. Comparison of the parabola and objective contributions to the total signal detected in three different tissues imaged in vivo.

Figure2

(Top Row), Maximum projection TPFM of GFP labeled microglia from a mouse brain (100 individual slices imaged at 1.5 μm intervals). The Combined (total epiTED system) and Parabola images were acquired separately as detailed in the Materials and Methods section. The objective image was calculated from the percent difference between the Combined and Parabola difference. The scale bar represents 30μm. (Middle Row), Single plane image of di-8- ANNEPS labeled blood vessels in an intact kidney imaged in vivo. The objective image was calculated as described above. The scale bar represents 60μm. (Bottom Row) Single plane in vivo image captured in the skeletal muscle of a mouse expressing DsRed under the actin promoter. The scale bar represents 30μm.The false color scalebar to the right of the image shows increasing intensity values from the bottom up (blue through white). Other details of image processing can be found in the Materials and Methods section.