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cDNA from adult female B. malayi worms was used as the template in PCR reactions. The relative location of the primers (FP, RP) used to detect the intergenic region is shown. (B) Agarose gel showing PCR product resulting from amplification of intergenic region between ribA and virB8-1. Genomic DNA, water, and reverse transcriptase-minus (RT−) samples were included as controls.
