Figure 7. wBmxR1 and wBmxR2 positively regulate the transcription of ribA lacZ reporter.

β–galactosidase assays were used to measure the transcriptional activities of lacZ reporter constructs. E. coli strain C2566 transformed with the reporter plasmid and protein expression vector pET21a, or pwBmxR1, or pwBmxR2 were tested. β–galactosidase assays were performed on induced (IPTG) and un-induced samples. Miller units are shown as mean ± standard deviations from 3 replica experiments. The ribA promoter region containing 400 bp (A) or 20 bp minimal binding sequence (B) were fused to a promoter-less lacZ and cloned into low copy plasmid pACYC184 to generate the reporter plasmid.