Table 2. Primers used to construct expression plasmids.

Protein	Primer Direction	Sequence 5′ to 3′	Size (bp)
wBmxR1	Forward	gagaCATATGGAAATAATATCATTAAACAATAACTCGTGC	297
	Reverse	gagaCTCGAGTGGAGATCCAACTTCACGTTTAACC
wBmxR2	Forward	ggaaCATATGCAAAATTCACAATTAAATTATCCTATAAC	279
	Reverse	gagaCTCGAGTTGTATGTTATGCTGAGAATTATGTTTTATTTTC
VirB8-1_His6	Forward	ttttCATATGCCGTTCGTTATAGAAATTGAAAAAAAATCAG	684
	Reverse	ttttCTCGAGTACATATTCATCATCTACCCTATAAGAAGTAAC
VirB8-1	Forward	ttttCATATGCCGTTCGTTATAGAAATTGAAAAAAAATCAG	681
	Reverse	ttttCTCGAGCTATACATATTCATCATCTACCCTATAAGAAGT
ΔTMVirB8-1_His6	Forward	ttttCTCGAGCTATACATATTCATCATCTACCCTATAAGAAGT	492
	Reverse	ttttCTCGAGTACATATTCATCATCTACCCTATAAGAAGTAAC

Restriction enzyme sites are underlined.

Additional nucleotides added to facilitate restriction enzyme digestion are shown in lower case.