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. 2012 Dec 10;7(12):e51661. doi: 10.1371/journal.pone.0051661

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© 2012 Badr et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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MM cells were untreated (0) or treated with 25 ng/ml of NP, 25 ng/ml of WEV and 10 ng/ml of WEV+NP for 12 hours, and the surface expression of CXCR4 and CD54 was analyzed by flow cytometry using PE-conjugate mAbs. Histograms were gated based on viable cells, and one representative experiment is shown with the surface expression of CXCR4 (A&B) and CD54 (C&D) on U266 and RPMI 8226 cells, respectively. The results for the MFI of CXCR4 (E) and CD54 (F) expressed on primary MM cells isolated from patients with multiple myeloma that were untreated (0), or treated with NP, WEV and WEV+NP are shown (n = 12).