Figure 5. Effects of autocrine IL-2 and IFNγ on function of Clone 4 cells in the tumor microenvironment. 8–9 week old RIP-Tag2-HA mice were immunized with peptide, polyI:C in IFA and Clone 4 or Clone 4 IL-2^−/− (3x10⁴) were injected i.v.. One group receiving Clone 4 cells was also injected with IFNγ neutralizing antibodies at days 4,5 and 6. (A–D) CD8⁺Thy1.1⁺ cells from pancreata were analyzed at day 7 by flow cytometry to assess the number of cells in the pancreas, the percentage of cells exhibiting granzyme B, the expression level of Bim and the percentage of dividing cells. Cumulative data are shown from 3 experiments with 2–3 mice per group per experiment. (E) Histograms are representative of 3 independent experiments with 3 mice per group. Isotype control = grey line, Clone 4 = black line, Clone 4 IL2^-/- = dashed line, Clone 4 + anti-IFNγ = dotted line.