Figure 1. Effects of LC treatment on tumor growth and survival. C57BL/6 mice (n = 5 per group) were injected with 2.5 X 10⁵ MCA205 cells s.c. and 3 d later, mice were treated once weekly with liposomal clodronate (LC) or a control liposomal preparation (L-PBS).. In (A), tumor growth was measured every 2–4 d and growth curves (mean tumor size (mm²), ± SEM) were generated. Tumor growth was significantly reduced (***p < 0.001) in the LC-treated mice, compared with control or L-PBS treated mice, as assessed by ANOVA. Similar results were obtained in 3 additional experiments. In (B), the effects of LC treatment on survival times were assessed. Survival times were significantly longer (p = 0.0032) in LC-treated mice, compared with L-PBS or control mice, as assessed by Kaplan-Meier curve and Logrank test. In (C), tumor growth curves were generated for s.c. injected CT-26 cells treated with either LC (i.v.), LC (i.p.) or L-PBS (i.v.). Tumor growth was significantly reduced (**p < 0.01) in LC-treated mice compared with L-PBS treated mice, as assessed by ANOVA.