Table I.
Effect of HS on Ca2+-dependent luminescence of purified aequorin
HS | 45°C | 50°C |
---|---|---|
min | counts s−1 | |
0 | 107,160 ± 987 | 108,317 ± 741 |
20 | 105,516 ± 1662 | 109,200 ± 1008 |
40 | 103,673 ± 1280 | 109,788 ± 860 |
60 | 106,831 ± 1036 | 109,928 ± 1788 |
Solutions of purified aequorin were heat treated at a given temperature for the times shown and then discharged with excess CaCl2. The values are means ± se of five measurements.