Table II.

Effects of prior HS of transgenic tobacco seedlings (MAQ 2.4) on their subsequent response to cold shock and wind signaling

HS	25°C, Control	39°C, 35 min	43°C, 35 min	47°C, 30 min
	pCa
Background	7.0  ± 0.02	7.01  ± 0.01	7.0  ± 0.02	7.0  ± 0.01
Cold-shock-induced increase in [Ca2+]cyt	6.0  ± 0.1	6.1  ± 0.1	5.9  ± 0.07	6.4  ± 0.03
Wind-stimulus-induced increase in [Ca2+]cyt	6.3  ± 0.03		6.1  ± 0.03

Three tobacco seedlings in a cuvette were first heat shocked in a waterbath at a given temperature for the given time as shown in Table II, taken out, and cooled down for 5 min at 25°C. Then the cuvette was placed into the sample chamber of the chemiluminometer. For cold shock 1 mL of ice-cold water was injected gently into the cuvette and the cold shock-induced Ca²⁺-dependent luminescence of the seedlings was recorded numerically for 15 s. For wind stimulation, 10 mL of air was injected rapidly over the seedlings by a port in the sample chamber with a syringe and the wind-induced luminescence of the seedlings was measured numerically for 15 s. Remaining aequorin was estimated at the end of the treatment and cytosolic pCa was calculated. The values are means ± se of 6 to 10 replicates.