Figure 4. T_CM persist to greater extent than T_EM in both Rag1^−/− and Rag.2C mice.

Rag1^−/− (filled symbols) and Rag.2C (open symbols) mice bearing well-healed K5mOVA grafts were adoptively transferred with 10⁶ T_CM (circles, black lines on histogram) or T_EM (squares, grey lines on histogram). 45 days following transfer spleen (panels A&B), pooled inguinal, brachial, axillary and mesenteric lymph nodes (panels C&D) and liver (panels E&F) were harvested and cells stained for CD8, Vα2, and CD62L. A, C and E. CD8⁺ Vα2⁺ cells as percent of total cells in each organ. n=4per group, **p<0.01 when comparing Rag1^−/− to Rag1^−/− and Rag.2C to Rag.2C, ns = not significant; analysed by one-way ANOVA analysis with post-hoc multiple comparison test. B, D and F. Representative CD62L expression of CD8⁺ Vα2⁺ CD44⁺ in various organs analysed by flow cytometry 45 days following transfer into Rag1−/− mice.