Figure 6. Loss of Rictor increases sensitivity of MEFs to UVB-induced apoptosis.
Rictor wild-type (+/+) and knock-out (-/-) MEFs were exposed to UVB (50mJ/cm2) at 70% confluence. A, Immunoblot analysis of mTORC1 and mTORC2 activation markers in cells exposed to UVB. B, MTS cell viability (mean ± SEM) at 24 h post UVB exposure; ** p<0.01. C, Annexin-V flow cytometry (mean ± SEM) at 24-h post UVB exposure. D, Immunoblot analysis of caspase-3 and quantification (mean ± SEM); * p<0.05. All data are representative from 2-4 independent experiments.