Figure 2.

Homogeneity of the lmw Hsp purified from intact chloroplasts isolated from heat-stressed tomato plants. Coomassie-blue stain (A) and immunoblot (western) (B) of protein samples eluted from an Ab_met column. Eluted proteins were fractionated by gel electrophoresis. One-half of the gel (two lanes) was stained with Coomassie blue to detect all proteins present. Proteins in unstained replicate lanes were electroblotted to membranes and probed with Ab_met. The locations of molecular mass markers (M), which were included on both the Coomassie-blue-stained gel and the immunoblot, are indicated.