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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Cell Immunol. 2012 Oct 1;279(1):46–52. doi: 10.1016/j.cellimm.2012.09.004

Figure 3. Phagosomal NKLAM expression decreases during maturation.

Figure 3

WT or NKLAM-KO BMDM were stimulated with LPS/IFNγ for 18 hr. The cells were incubated with IgG-opsonized magnetic beads at 4°C for 10 min to synchronize bead binding. Ingestion was initiated by incubating the cells at 37°C. After incubation for the times indicated, the phagosomes were isolated as described in Materials and Methods. Phagosome lysates were normalized by protein and immunoblotted for NKLAM and phagosome marker cathepsin D.