Table 1.
Synthetic Spider Silk Protein Sequences with Their Gene and Protein Sizes Listeda
protein sequence | DNA size (bp) | protein Mw (kDa) | |
---|---|---|---|
elasticity cassette (E) | GPGGYGPGQQ | 30 | |
strength 8 cassette (S8) | GPGGPSGPGSA8 | 54 | |
(MaSp1)24 | (GGAGQGGYGGLGSQGAGRGGLGGQGAGA6)24 | 2376 | 66 (62) |
(MaSp2)16 | (GPGGYGPGQQGPGGYGPGQQGPSGPGSA8)16 | 1680 | 48 (47) |
chimeric (148)8 | (PGG + MaSp1 + LAGPGQQG + E4 + S8)8 | 2448 | 70 (68) |
chimeric (188)6 | (PGG + MaSp1 + LAGPGQQG + E8 + S8)6 | 2556 | 75 (73) |
For protein molecular weight, numbers in the parentheses are calculated Mw. His-tag sequence at N-terminal of the proteins is not shown and calculated.