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. Author manuscript; available in PMC: 2013 Dec 15.
Published in final edited form as: J Immunol. 2012 Nov 9;189(12):5667–5681. doi: 10.4049/jimmunol.1201661

Figure 6. Significantly higher number of proliferating B cells in B6.Sle1b female mice.

Figure 6

(A) Spleen sections from 6–9 month old B6 and B6.Sle1b mice injected with BrdU 12 hours and 1–2 hours prior to sacrifice were stained for anti-IgD (blue) and anti-BrdU (brown). Original magnification of the images was 100× (left) and 200× (right). (B) Semi-quantitative analysis of the number of BrdU+ cells in follicular-GC (F-GC, gray bars) versus extrafollicular (EF, white bars) regions. BrdU+ cells were counted in two randomly picked areas per spleen at ×100 original magnification. Five B6 and 5 B6.Sle1b female mice were analyzed. (C) Spleen sections obtained from SRBC-immunized 5–6 week old female B6 and B6.Sle1b mice were analyzed for BrdU+ cells inside the GCs as described in A. Parallel spleen sections were obtained from 6–9 month old female B6 and B6.Sle1b mice and stained for either GL7 (green) and anti-BrdU (red) as shown in (D) or anti-CD4 (green) and anti-BrdU (red) as shown in (E). (F) Spleen sections from 6–9 month old female B6 and B6.Sle1b mice were stained for anti-IgD (blue), GL7 (green), and anti-Ki67 (red). Original magnification of the images in C-E was 200×. These data were generated from at least 5 female mice of each genotype.