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. Author manuscript; available in PMC: 2013 Dec 7.
Published in final edited form as: Cell. 2012 Nov 21;151(6):1214–1228. doi: 10.1016/j.cell.2012.11.009

Figure 3. UpSET interacts with an HDAC complex.

Figure 3

(A) UpSET-bound regions are enriched for the Histone deacetylase Rpd3 and its associated cofactor Sin3. Box plot analysis of Rpd3 and Sin3 DamID signal overlapping with UpSET bound regions.

(B) Browser view of chromosome 2L highlighting the overlapping DamID signal for UpSET, Rpd3 and Sin3.

(C) Venn diagram showing the overlap among regions bound by UpSET, Rpd3, and Sin3. UpSET overlaps with only half of the loci co-bound by Rpd3 and Sin3.

(D–G) UpSET co-localizes with Sin3 and Rpd3 on chromosomes. Polytene chromosome sets stained with a mixture of antibodies to NH3- UpSET (D’-D”, E’-E”, F–G; green) and Rpd3 (D, D”, G; red) or Sin3 (E, E”, F; red). DNA was counterstained with DAPI (blue). (F–G) Higher magnification views showing co-localization of UpSET with Rpd3 (G) or Sin3 (F).

(H) UpSET interacts with Rpd3 and Sin3. Co-immunoprecipitation of UpSET with Rpd3 and Sin3 from Kc nuclear extracts. Arrows indicate the specified protein. 20% input is shown.