Table 1. upSET.
mutants are female sterile, increase Polycomb phenotypes, and reduce Trithorax-associated homeotic transformations.
| Lack of UpSET causes female sterility
| |||||
|---|---|---|---|---|---|
| Genotype
|
|||||
| Female | Male | Sterile females % | No. sterile females N=50 | Rescue % | No. Egg |
| +/+ | +/+ | 0 | 0 | n/a | 1536 |
| +/ upSETe00365 | +/+ | 0 | 0 | n/a | 1330 |
| upSETe00365/ upSETe00365 | +/+ | 98 | 49 | n/a | 5 |
| BAC1X a; upSETe00365/ upSETe00365 | +/+ | 26 | 13 | 72 | 1035 |
| BAC2X b; upSETe00365/ upSETe00365 | +/+ | 5 | 3 | 93 | 1231 |
| mMLL5 c; upSETe00365/ upSETe00365 | +/+ | 78 | 39 | 20 | 408 |
| upSET enhances Pc phenotypes. | |||
|---|---|---|---|
| % with enhanced phenotype e, f (n)
|
|||
| Genotype*: | + | upSETe00365 | upSETMB8950 g |
| +/+; +/* d | 0 (350) | 0 (303) | 0 (239) |
| Pc3 /+; +/* e | 40 (403) | 89 (495) | 76 (184) |
| Pc1 Abd-B Mc /+; +/* e | 33 (320) | 75 (256) | 75 (122) |
| brm2 trxE2 /+ ; +/* f | 56 (465) | 9 (327) | 4 (409) |
a
BAC1X= w; P[acman](attA CH322.187J08 attB)/CyO.
b
BAC2X= w; P[acman](attA CH322.187J08 attB)/ P[acman](attA CH322.187J08 attB)
c
mMLL5= w;UAS_mMLL5/ActGAL4
d
wildtype is OregonR
e
For the Pc alleles Pc3 and Pc1, males of the genotypes indicated in the first column were scored for the presence of a sex comb on the 2nd and/or third leg (usually found only on the first leg).
f
brm2 trxE2 was scored for the presence of an apical bristle on the third leg (usually found on the second leg).
g
See also Figure S7A n/a = not applicable